What is the difference between gfp and yfp

However, their applications in molecular biology are the same. GFP green fluorescent protein is a bioluminescent polypeptide protein naturally occurs in the jellyfish, Aequorea Victoria , and many other marine organisms.

In Aequorea Victoria , it is known as aequorin and emits fluorescence when it gets exposed to ranges from blue to ultraviolet light. That means; GFP completely absorbs blue light nm or the nm light in the long UV range and emit green light nm. Figure 1: Aequorea Victoria. The GFP protein contains amino acids and the size of the protein is It folds to form the shape of a beta-barrel. Here, the part of the protein which makes it fluorescent is formed from the conjugation of the main chain atoms, Ser65, Tyr66, and Gly67, forming the highly conjugated, planar p-hydroxybenzylideneimidazolinone chromophore in the presence of oxygen.

The chromophore is packed inside the beta-barrel structure, protecting the chromophore from quenching through paramagnetic oxygen, water dipoles or cis-trans isomerization. Also, the non-covalent interactions of the chromophore with the neighboring molecules enhances its spectral properties. It is a mechanism which describes the energy transfer between 2 chromophores. Difference Between Similar Terms and Objects. MLA 8 Kaushik, Nimisha.

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It is a mutant of the originally derived Green Fluorescent Protein from the jellyfish Aequorea Victoria. Yellow fluorescent protein YFP is a genetic mutant of green fluorescent protein GFP originally derived from the jellyfish Aequorea victoria. GFP is unique among fluorescent proteins in that its fluorophore is not a seperately synthesized prostethic group but composed of modified amino acid residues within the polypeptide chain.

Green-fluorescent protein GFP is the origin of the green bioluminescence color exhibited by several marine hydrozoans and anthozoans. Flow cytometry and fluorescent microscopy are two conventional tools to detect the GFP signal; flow cytometry is an effective and sensitive technique to quantitatively analyze fluorescent intensity, while fluorescent microscopy can visualize the subcellular location and expression of GFP.

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